THF1 mutations lead to increased basal and wound‐induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI1 signaling in Arabidopsis

Xuemin Wang

doi:10.1111/jipb.12177

THF1 mutations lead to increased basal and wound‐induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI1 signaling in Arabidopsis

Xuemin Wang

University of Missouri-St. Louis

Research output: Contribution to journal › Article › peer-review

Abstract

<div class="line" id="line-15"> Mutants defective in chloroplast development or photosynthesis are liable to accumulate higher levels of anthocyanin in photo&hyphen;oxidative stress. However, regulatory mechanisms of anthocyanin biosynthesis in the mutants remain unclear. Here, we investigated the mechanism by which the deletion of  thylakoid formation1  ( THF1  ) leads to an increased level of anthocyanin in  Arabidopsis thaliana  L. Physiological and genetic evidence showed that the increased level of anthocyanin in  thf1  is dependent on coronatine&hyphen;insensitive1 (COI1) signaling. Our data showed that  thf1  had higher levels of basal α&hyphen;linolenic acid (α&hyphen;LeA), and methyl jasmonate (JA)&hyphen;induced α&hyphen;LeA and 12&hyphen;oxophytodienoic acid (OPDA) than the wild type (WT). Consistently, expression levels of phospholipase genes including  pPLAIIα  and  PLA&hyphen;Iγ1  were elevated in  thf1  . Furthermore, inhibition of lipase activity by bromoenol lactone, a specific inhibitor of plant pPLA, led to producing identical levels of anthocyanins in WT and  thf1  plants. Interestingly, OPDA biosynthesis was triggered by light illumination in isolated chloroplasts, indicating that new protein import into chloroplasts is not required for OPDA biosynthesis. Thus, we conclude that the elevated anthocyanin accumulation in  thf1  is attributed to an increase in JA levels. This JA&hyphen;mediated signaling to coordinate plant metabolism and growth in stress may be conserved in other photosensitive mutants. </div>

Original language	American English
Journal	Journal of Integrative Plant Biology
Volume	56
DOIs	https://doi.org/10.1111/jipb.12177
State	Published - 2014

Keywords

Anthocyanin
Arabidopsis
THF 1
jasmonates
photo‐oxidative stress
retrograde signaling

Disciplines

Biology
Botany

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@article{21e5e955185b4415b04bf3e8fa618ba0,

title = "THF1 mutations lead to increased basal and wound‐induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI1 signaling in Arabidopsis",

abstract = " Mutants defective in chloroplast development or photosynthesis are liable to accumulate higher levels of anthocyanin in photo&hyphen;oxidative stress. However, regulatory mechanisms of anthocyanin biosynthesis in the mutants remain unclear. Here, we investigated the mechanism by which the deletion of  thylakoid formation1  ( THF1  ) leads to an increased level of anthocyanin in  Arabidopsis thaliana  L. Physiological and genetic evidence showed that the increased level of anthocyanin in  thf1  is dependent on coronatine&hyphen;insensitive1 (COI1) signaling. Our data showed that  thf1  had higher levels of basal α&hyphen;linolenic acid (α&hyphen;LeA), and methyl jasmonate (JA)&hyphen;induced α&hyphen;LeA and 12&hyphen;oxophytodienoic acid (OPDA) than the wild type (WT). Consistently, expression levels of phospholipase genes including  pPLAIIα  and  PLA&hyphen;Iγ1  were elevated in  thf1  . Furthermore, inhibition of lipase activity by bromoenol lactone, a specific inhibitor of plant pPLA, led to producing identical levels of anthocyanins in WT and  thf1  plants. Interestingly, OPDA biosynthesis was triggered by light illumination in isolated chloroplasts, indicating that new protein import into chloroplasts is not required for OPDA biosynthesis. Thus, we conclude that the elevated anthocyanin accumulation in  thf1  is attributed to an increase in JA levels. This JA&hyphen;mediated signaling to coordinate plant metabolism and growth in stress may be conserved in other photosensitive mutants. ",

keywords = "Anthocyanin, Arabidopsis, THF 1, jasmonates, photo‐oxidative stress, retrograde signaling",

author = "Xuemin Wang",

note = "Yi Gan National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai, 200032 China School of Agricultural and Food Science, Zhejiang A&F University, Lin'an, 311300 China Search for more papers by this author Hong Li",

year = "2014",

doi = "10.1111/jipb.12177",

language = "American English",

volume = "56",

journal = "Journal of Integrative Plant Biology",

}

TY - JOUR

T1 - THF1 mutations lead to increased basal and wound‐induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI1 signaling in Arabidopsis

AU - Wang, Xuemin

N1 - Yi Gan National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai, 200032 China School of Agricultural and Food Science, Zhejiang A&F University, Lin'an, 311300 China Search for more papers by this author Hong Li

PY - 2014

Y1 - 2014

N2 - Mutants defective in chloroplast development or photosynthesis are liable to accumulate higher levels of anthocyanin in photo&hyphen;oxidative stress. However, regulatory mechanisms of anthocyanin biosynthesis in the mutants remain unclear. Here, we investigated the mechanism by which the deletion of  thylakoid formation1  ( THF1  ) leads to an increased level of anthocyanin in  Arabidopsis thaliana  L. Physiological and genetic evidence showed that the increased level of anthocyanin in  thf1  is dependent on coronatine&hyphen;insensitive1 (COI1) signaling. Our data showed that  thf1  had higher levels of basal α&hyphen;linolenic acid (α&hyphen;LeA), and methyl jasmonate (JA)&hyphen;induced α&hyphen;LeA and 12&hyphen;oxophytodienoic acid (OPDA) than the wild type (WT). Consistently, expression levels of phospholipase genes including  pPLAIIα  and  PLA&hyphen;Iγ1  were elevated in  thf1  . Furthermore, inhibition of lipase activity by bromoenol lactone, a specific inhibitor of plant pPLA, led to producing identical levels of anthocyanins in WT and  thf1  plants. Interestingly, OPDA biosynthesis was triggered by light illumination in isolated chloroplasts, indicating that new protein import into chloroplasts is not required for OPDA biosynthesis. Thus, we conclude that the elevated anthocyanin accumulation in  thf1  is attributed to an increase in JA levels. This JA&hyphen;mediated signaling to coordinate plant metabolism and growth in stress may be conserved in other photosensitive mutants.

AB - Mutants defective in chloroplast development or photosynthesis are liable to accumulate higher levels of anthocyanin in photo&hyphen;oxidative stress. However, regulatory mechanisms of anthocyanin biosynthesis in the mutants remain unclear. Here, we investigated the mechanism by which the deletion of  thylakoid formation1  ( THF1  ) leads to an increased level of anthocyanin in  Arabidopsis thaliana  L. Physiological and genetic evidence showed that the increased level of anthocyanin in  thf1  is dependent on coronatine&hyphen;insensitive1 (COI1) signaling. Our data showed that  thf1  had higher levels of basal α&hyphen;linolenic acid (α&hyphen;LeA), and methyl jasmonate (JA)&hyphen;induced α&hyphen;LeA and 12&hyphen;oxophytodienoic acid (OPDA) than the wild type (WT). Consistently, expression levels of phospholipase genes including  pPLAIIα  and  PLA&hyphen;Iγ1  were elevated in  thf1  . Furthermore, inhibition of lipase activity by bromoenol lactone, a specific inhibitor of plant pPLA, led to producing identical levels of anthocyanins in WT and  thf1  plants. Interestingly, OPDA biosynthesis was triggered by light illumination in isolated chloroplasts, indicating that new protein import into chloroplasts is not required for OPDA biosynthesis. Thus, we conclude that the elevated anthocyanin accumulation in  thf1  is attributed to an increase in JA levels. This JA&hyphen;mediated signaling to coordinate plant metabolism and growth in stress may be conserved in other photosensitive mutants.

KW - Anthocyanin

KW - Arabidopsis

KW - THF 1

KW - jasmonates

KW - photo‐oxidative stress

KW - retrograde signaling

UR - https://doi.org/10.1111/jipb.12177

U2 - 10.1111/jipb.12177

DO - 10.1111/jipb.12177

M3 - Article

VL - 56

JO - Journal of Integrative Plant Biology

JF - Journal of Integrative Plant Biology

ER -

THF1 mutations lead to increased basal and wound‐induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI1 signaling in Arabidopsis

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