TY - JOUR
T1 - Studying Bacterial Hydrophobicity and Biofilm Formation at Liquid–Liquid Interfaces Through Interfacial Rheology and Pendant Drop Tensiometry
AU - Inglis, Fredrik
AU - Fischer, P
AU - Böcker, L
AU - Rühs, P A
N1 - JavaScript is disabled on your browser. Please enable JavaScript to use all the features on this page. * We measured the transient elasticity at the interface through interfacial rheology. * Growing and non-growing bacteria form viscoelastic layers at water-oil interfaces. * Bacterial adsorption was quantified through tensiometry and rheology.
PY - 2014/5/1
Y1 - 2014/5/1
N2 - Bacterial adsorption to interfaces is a key factor in biofilm formation. One major limitation to understanding biofilm formation and development is the accurate measurement of bacterial cell adhesion to hydrophobic interfaces. With this study, bacterial attachment and biofilm growth over time at water–oil interface was monitored through interfacial rheology and tensiometry. Five model bacteria (Pseudomonas putida KT2442, Pseudomonas putida W2, Salmonella typhimurium, Escherichia coli, and Bacillus subtilis) were allowed to adsorb at the water–oil interface either in their non-growing or growing state. We found that we were able to observe the initial kinetics of bacterial attachment and the transient biofilm formation at the water–oil interface through interfacial rheology and tensiometry. Electrophoretic mobility measurements and bacterial adhesion to hydrocarbons (BATH) tests were performed to characterize the selected bacteria. To validate interfacial rheology and tensiometry measurements, we monitored biofilm formation utilizing both confocal laser scanning microscopy and light microscopy. Using this combination of techniques, we were able to observe the elasticity and tension development over time, from the first bacterial attachment up to biofilm formation.
AB - Bacterial adsorption to interfaces is a key factor in biofilm formation. One major limitation to understanding biofilm formation and development is the accurate measurement of bacterial cell adhesion to hydrophobic interfaces. With this study, bacterial attachment and biofilm growth over time at water–oil interface was monitored through interfacial rheology and tensiometry. Five model bacteria (Pseudomonas putida KT2442, Pseudomonas putida W2, Salmonella typhimurium, Escherichia coli, and Bacillus subtilis) were allowed to adsorb at the water–oil interface either in their non-growing or growing state. We found that we were able to observe the initial kinetics of bacterial attachment and the transient biofilm formation at the water–oil interface through interfacial rheology and tensiometry. Electrophoretic mobility measurements and bacterial adhesion to hydrocarbons (BATH) tests were performed to characterize the selected bacteria. To validate interfacial rheology and tensiometry measurements, we monitored biofilm formation utilizing both confocal laser scanning microscopy and light microscopy. Using this combination of techniques, we were able to observe the elasticity and tension development over time, from the first bacterial attachment up to biofilm formation.
UR - https://www.sciencedirect.com/science/article/pii/S0927776514000903
U2 - 10.1016/j.colsurfb.2014.02.023
DO - 10.1016/j.colsurfb.2014.02.023
M3 - Article
VL - 117
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -