Structure and function of the catalytic site mutant Asp 99 Asn of phospholipase A2: absence of the conserved structural water.

Amarendra Kumar; Chandra Sekharudu; Boopathy Ramakrishnan; Cynthia M. Dupureur; Hongxin Zhu; Ming-Daw Tsai; Muttaiya Sundaralingam

doi:10.1002/pro.5560031121

Structure and function of the catalytic site mutant Asp 99 Asn of phospholipase A2: absence of the conserved structural water.

Amarendra Kumar
, Chandra Sekharudu
, Boopathy Ramakrishnan
, Cynthia M. Dupureur
, Hongxin Zhu
, Ming-Daw Tsai
, Muttaiya Sundaralingam

Research output: Contribution to journal › Article › peer-review

Original language	American English
Journal	Protein Science
Volume	3
DOIs	https://doi.org/10.1002/pro.5560031121
State	Published - Nov 1 1994
Externally published	Yes

Disciplines

Biochemistry

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@article{92467db22c484e0097be0e7139ae1ca7,

title = "Structure and function of the catalytic site mutant Asp 99 Asn of phospholipase A2: absence of the conserved structural water.",

author = "Amarendra Kumar and Chandra Sekharudu and Boopathy Ramakrishnan and Dupureur, \{Cynthia M.\} and Hongxin Zhu and Ming-Daw Tsai and Muttaiya Sundaralingam",

note = "To probe the role of the Asp-99 ... His-48 pair in phospholipase A2 (PLA2) catalysis, the X-ray structure and kinetic characterization of the mutant Asp-99-->Asn-99 (D99N) of bovine pancreatic PLA2 was undertaken. Crystals of D99N belong to the trigonal space group P3(1)21 and were isomorphous to the wild type (WT) (Noel JP et al., 1991, Biochemistry 30:11801-11811).",

year = "1994",

month = nov,

day = "1",

doi = "10.1002/pro.5560031121",

language = "American English",

volume = "3",

journal = "Protein Science",

}

TY - JOUR

T1 - Structure and function of the catalytic site mutant Asp 99 Asn of phospholipase A2: absence of the conserved structural water.

AU - Kumar, Amarendra

AU - Sekharudu, Chandra

AU - Ramakrishnan, Boopathy

AU - Dupureur, Cynthia M.

AU - Zhu, Hongxin

AU - Tsai, Ming-Daw

AU - Sundaralingam, Muttaiya

N1 - To probe the role of the Asp-99 ... His-48 pair in phospholipase A2 (PLA2) catalysis, the X-ray structure and kinetic characterization of the mutant Asp-99-->Asn-99 (D99N) of bovine pancreatic PLA2 was undertaken. Crystals of D99N belong to the trigonal space group P3(1)21 and were isomorphous to the wild type (WT) (Noel JP et al., 1991, Biochemistry 30:11801-11811).

PY - 1994/11/1

Y1 - 1994/11/1

UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2142646/

U2 - 10.1002/pro.5560031121

DO - 10.1002/pro.5560031121

M3 - Article

VL - 3

JO - Protein Science

JF - Protein Science

ER -

Structure and function of the catalytic site mutant Asp 99 Asn of phospholipase A2: absence of the conserved structural water.

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