Spectral Unmixing of Multicolored Bioluminescence Emitted from Heterogeneous Biological Sources

Seth T. Gammon; W. Matthew Leevy; Shimon Gross; George W. Gokel; David Piwnica-Worms

doi:10.1021/ac051999h

Spectral Unmixing of Multicolored Bioluminescence Emitted from Heterogeneous Biological Sources

Seth T. Gammon, W. Matthew Leevy, Shimon Gross, George W. Gokel, David Piwnica-Worms

Research output: Contribution to journal › Article › peer-review

Original language	American English
Journal	Analytical Chemistry
Volume	78
DOIs	https://doi.org/10.1021/ac051999h
State	Published - Jan 3 2006
Externally published	Yes

Disciplines

Analytical Chemistry

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10.1021/ac051999hLicense: Unspecified

Cite this

@article{d466554c9bb04838906e9d63af6d1e0f,

title = "Spectral Unmixing of Multicolored Bioluminescence Emitted from Heterogeneous Biological Sources",

author = "Gammon, \{Seth T.\} and Leevy, \{W. Matthew\} and Shimon Gross and Gokel, \{George W.\} and David Piwnica-Worms",

note = "A wide variety of bioluminescent luciferase proteins are available for use in transcriptional or biochemical reporter assays. However, spectral overlap normally prevents them from being monitored simultaneously. To address this problem, a Java plug-in for ImageJ was written to deconvolute bioluminescent images composed of signals from multiple luciferases.",

year = "2006",

month = jan,

day = "3",

doi = "10.1021/ac051999h",

language = "American English",

volume = "78",

journal = "Analytical Chemistry",

}

TY - JOUR

T1 - Spectral Unmixing of Multicolored Bioluminescence Emitted from Heterogeneous Biological Sources

AU - Gammon, Seth T.

AU - Leevy, W. Matthew

AU - Gross, Shimon

AU - Gokel, George W.

AU - Piwnica-Worms, David

N1 - A wide variety of bioluminescent luciferase proteins are available for use in transcriptional or biochemical reporter assays. However, spectral overlap normally prevents them from being monitored simultaneously. To address this problem, a Java plug-in for ImageJ was written to deconvolute bioluminescent images composed of signals from multiple luciferases.

PY - 2006/1/3

Y1 - 2006/1/3

UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615584/

U2 - 10.1021/ac051999h

DO - 10.1021/ac051999h

M3 - Article

VL - 78

JO - Analytical Chemistry

JF - Analytical Chemistry

ER -

Spectral Unmixing of Multicolored Bioluminescence Emitted from Heterogeneous Biological Sources

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