Rice sulfoquinovosyltransferase SQD2.1 mediates flavonoid glycosylation and enhances tolerance to osmotic stress

Xuemin Wang

doi:10.1111/pce.13554

Rice sulfoquinovosyltransferase SQD2.1 mediates flavonoid glycosylation and enhances tolerance to osmotic stress

Xuemin Wang

University of Missouri-St. Louis

Research output: Contribution to journal › Article › peer-review

Abstract

<div class="line" id="line-15"> Sulfoquinovosyltransferase 2 (SQD2) catalyses the final step in the sulfoquinovosyldiacylglycerol (SQDG) biosynthetic pathway. It is involved in the phosphate starvation response. Here, we show that rice SQD2.1 has dual activities catalysing SQDG synthesis and flavonoid glycosylation.  SQD2.1  null mutants ( sqd2.1  ) in rice had decreased levels of glycosidic flavonoids, particularly apigenin 7&hyphen; O &hyphen;glucoside (A7G), whereas these metabolites were increased in rice plants overexpressing  SQD2.1  . The  sqd2.1  mutants and  SQD2.1  overexpressing lines showed reduced and enhanced, respectively, tolerance to salinity and drought. Treating the  sqd2.1  mutants with A7G decreased oxidative damage and restored stress tolerance to the wild&hyphen;type levels. These findings demonstrate that SQD2.1 has a novel function in the glycosylation of flavonoids that is required for osmotic stress tolerance in rice. The novel activity of SQD2.1 in the production of glycosidic flavonoids improves scavenging of reactive oxygen species and protects against excessive oxidation. </div>

Original language	American English
Journal	Plant, Cell & Environment
Volume	42
DOIs	https://doi.org/10.1111/pce.13554
State	Published - 2019

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Botany

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@article{5ccf44f8f8f04049af86e2b53af33915,

title = "Rice sulfoquinovosyltransferase SQD2.1 mediates flavonoid glycosylation and enhances tolerance to osmotic stress",

abstract = " Sulfoquinovosyltransferase 2 (SQD2) catalyses the final step in the sulfoquinovosyldiacylglycerol (SQDG) biosynthetic pathway. It is involved in the phosphate starvation response. Here, we show that rice SQD2.1 has dual activities catalysing SQDG synthesis and flavonoid glycosylation.  SQD2.1  null mutants ( sqd2.1  ) in rice had decreased levels of glycosidic flavonoids, particularly apigenin 7&hyphen; O &hyphen;glucoside (A7G), whereas these metabolites were increased in rice plants overexpressing  SQD2.1  . The  sqd2.1  mutants and  SQD2.1  overexpressing lines showed reduced and enhanced, respectively, tolerance to salinity and drought. Treating the  sqd2.1  mutants with A7G decreased oxidative damage and restored stress tolerance to the wild&hyphen;type levels. These findings demonstrate that SQD2.1 has a novel function in the glycosylation of flavonoids that is required for osmotic stress tolerance in rice. The novel activity of SQD2.1 in the production of glycosidic flavonoids improves scavenging of reactive oxygen species and protects against excessive oxidation. ",

author = "Xuemin Wang",

note = "Rice SQD2.1 possesses dual activities catalysing SQDG synthesis and flavonoid glycosylation, and the activity of glycosylating flavonoids, not its SQDG synthesis, improves plant tolerance to salt and...",

year = "2019",

doi = "10.1111/pce.13554",

language = "American English",

volume = "42",

journal = "Plant, Cell & Environment",

}

TY - JOUR

T1 - Rice sulfoquinovosyltransferase SQD2.1 mediates flavonoid glycosylation and enhances tolerance to osmotic stress

AU - Wang, Xuemin

N1 - Rice SQD2.1 possesses dual activities catalysing SQDG synthesis and flavonoid glycosylation, and the activity of glycosylating flavonoids, not its SQDG synthesis, improves plant tolerance to salt and...

PY - 2019

Y1 - 2019

N2 - Sulfoquinovosyltransferase 2 (SQD2) catalyses the final step in the sulfoquinovosyldiacylglycerol (SQDG) biosynthetic pathway. It is involved in the phosphate starvation response. Here, we show that rice SQD2.1 has dual activities catalysing SQDG synthesis and flavonoid glycosylation.  SQD2.1  null mutants ( sqd2.1  ) in rice had decreased levels of glycosidic flavonoids, particularly apigenin 7&hyphen; O &hyphen;glucoside (A7G), whereas these metabolites were increased in rice plants overexpressing  SQD2.1  . The  sqd2.1  mutants and  SQD2.1  overexpressing lines showed reduced and enhanced, respectively, tolerance to salinity and drought. Treating the  sqd2.1  mutants with A7G decreased oxidative damage and restored stress tolerance to the wild&hyphen;type levels. These findings demonstrate that SQD2.1 has a novel function in the glycosylation of flavonoids that is required for osmotic stress tolerance in rice. The novel activity of SQD2.1 in the production of glycosidic flavonoids improves scavenging of reactive oxygen species and protects against excessive oxidation.

AB - Sulfoquinovosyltransferase 2 (SQD2) catalyses the final step in the sulfoquinovosyldiacylglycerol (SQDG) biosynthetic pathway. It is involved in the phosphate starvation response. Here, we show that rice SQD2.1 has dual activities catalysing SQDG synthesis and flavonoid glycosylation.  SQD2.1  null mutants ( sqd2.1  ) in rice had decreased levels of glycosidic flavonoids, particularly apigenin 7&hyphen; O &hyphen;glucoside (A7G), whereas these metabolites were increased in rice plants overexpressing  SQD2.1  . The  sqd2.1  mutants and  SQD2.1  overexpressing lines showed reduced and enhanced, respectively, tolerance to salinity and drought. Treating the  sqd2.1  mutants with A7G decreased oxidative damage and restored stress tolerance to the wild&hyphen;type levels. These findings demonstrate that SQD2.1 has a novel function in the glycosylation of flavonoids that is required for osmotic stress tolerance in rice. The novel activity of SQD2.1 in the production of glycosidic flavonoids improves scavenging of reactive oxygen species and protects against excessive oxidation.

UR - https://doi.org/10.1111/pce.13554

U2 - 10.1111/pce.13554

DO - 10.1111/pce.13554

M3 - Article

VL - 42

JO - Plant, Cell & Environment

JF - Plant, Cell & Environment

ER -

Rice sulfoquinovosyltransferase SQD2.1 mediates flavonoid glycosylation and enhances tolerance to osmotic stress

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