Regulation of V‐nitrogenase genes in Anabaena variabilis by RNA processing and by dual repressors

Brenda S. Pratte; Ryan Sheridan; Jessie A. James; Teresa Thiel

doi:10.1111/mmi.12197

Regulation of V‐nitrogenase genes in Anabaena variabilis by RNA processing and by dual repressors

Brenda S. Pratte, Ryan Sheridan, Jessie A. James, Teresa Thiel

University of Missouri-St. Louis

Research output: Contribution to journal › Article › peer-review

Abstract

<div class="line" id="line-15"> A nabaena variabilis  &emsp14;ATCC 29413 fixes nitrogen in specialized cells called heterocysts using either a Mo &hyphen;nitrogenase or a V &hyphen;nitrogenase. V &hyphen;nitrogenase structural genes,  vnfDGK  , as well as  vnfEN  form an operon with  ava4025  , located upstream of  vnfDG  that is repressed by fixed nitrogen and by Mo . The  ava4025  &hyphen; vnfDGKEN  operon is under the control of a Mo &hyphen;repressible promoter located nearly 600 bp upstream of  ava4025  . Levels of  vnfDG  transcript were about 500&hyphen;fold higher than  ava4025  , the first gene of the operon. This may be the result of RNA processing at a site 87 bp upstream of  vnfDG  that was initially identified as the transcription start site. A strain with a deletion in the coding region of  ava4025  grew diazotrophically with Mo or with V . Two similar proteins, VnfR 1 and VnfR 2, whose genes are located some distance from the  ava4025  &hyphen; vnfDGKEN  operon, each repressed transcription from the  ava4025  &hyphen; vnfDGKEN  promoter and a mutant lacking both VnfR 1 and VnfR 2 made the V &hyphen;nitrogenase in the presence of Mo . Overexpression of the V &hyphen;nitrogenase in the double  vnfR1 vnfR2  mutant resulted in decreased activity of the Mo &hyphen;nitrogenase. VnfR 1 bound specifically,  in vitro  , to a region upstream of the  ava4025  promoter. </div>

Original language	American English
Journal	Molecular Microbiology
Volume	88
DOIs	https://doi.org/10.1111/mmi.12197
State	Published - Jan 4 2013

Disciplines

Genetics
Biology
Molecular Biology

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title = "Regulation of V‐nitrogenase genes in Anabaena variabilis by RNA processing and by dual repressors",

abstract = " A nabaena variabilis  &emsp14;ATCC 29413 fixes nitrogen in specialized cells called heterocysts using either a Mo &hyphen;nitrogenase or a V &hyphen;nitrogenase. V &hyphen;nitrogenase structural genes,  vnfDGK  , as well as  vnfEN  form an operon with  ava4025  , located upstream of  vnfDG  that is repressed by fixed nitrogen and by Mo . The  ava4025  &hyphen; vnfDGKEN  operon is under the control of a Mo &hyphen;repressible promoter located nearly 600 bp upstream of  ava4025  . Levels of  vnfDG  transcript were about 500&hyphen;fold higher than  ava4025  , the first gene of the operon. This may be the result of RNA processing at a site 87 bp upstream of  vnfDG  that was initially identified as the transcription start site. A strain with a deletion in the coding region of  ava4025  grew diazotrophically with Mo or with V . Two similar proteins, VnfR 1 and VnfR 2, whose genes are located some distance from the  ava4025  &hyphen; vnfDGKEN  operon, each repressed transcription from the  ava4025  &hyphen; vnfDGKEN  promoter and a mutant lacking both VnfR 1 and VnfR 2 made the V &hyphen;nitrogenase in the presence of Mo . Overexpression of the V &hyphen;nitrogenase in the double  vnfR1 vnfR2  mutant resulted in decreased activity of the Mo &hyphen;nitrogenase. VnfR 1 bound specifically,  in vitro  , to a region upstream of the  ava4025  promoter. ",

author = "Pratte, {Brenda S.} and Ryan Sheridan and James, {Jessie A.} and Teresa Thiel",

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year = "2013",

month = jan,

day = "4",

doi = "10.1111/mmi.12197",

language = "American English",

volume = "88",

journal = "Molecular Microbiology",

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TY - JOUR

T1 - Regulation of V‐nitrogenase genes in Anabaena variabilis by RNA processing and by dual repressors

AU - Pratte, Brenda S.

AU - Sheridan, Ryan

AU - James, Jessie A.

AU - Thiel, Teresa

N1 - Enter your email address below. If your address has been previously registered, you will receive an email with instructions on how to reset your password. If you don't receive an email, you should register as a new user

PY - 2013/1/4

Y1 - 2013/1/4

N2 - A nabaena variabilis  &emsp14;ATCC 29413 fixes nitrogen in specialized cells called heterocysts using either a Mo &hyphen;nitrogenase or a V &hyphen;nitrogenase. V &hyphen;nitrogenase structural genes,  vnfDGK  , as well as  vnfEN  form an operon with  ava4025  , located upstream of  vnfDG  that is repressed by fixed nitrogen and by Mo . The  ava4025  &hyphen; vnfDGKEN  operon is under the control of a Mo &hyphen;repressible promoter located nearly 600 bp upstream of  ava4025  . Levels of  vnfDG  transcript were about 500&hyphen;fold higher than  ava4025  , the first gene of the operon. This may be the result of RNA processing at a site 87 bp upstream of  vnfDG  that was initially identified as the transcription start site. A strain with a deletion in the coding region of  ava4025  grew diazotrophically with Mo or with V . Two similar proteins, VnfR 1 and VnfR 2, whose genes are located some distance from the  ava4025  &hyphen; vnfDGKEN  operon, each repressed transcription from the  ava4025  &hyphen; vnfDGKEN  promoter and a mutant lacking both VnfR 1 and VnfR 2 made the V &hyphen;nitrogenase in the presence of Mo . Overexpression of the V &hyphen;nitrogenase in the double  vnfR1 vnfR2  mutant resulted in decreased activity of the Mo &hyphen;nitrogenase. VnfR 1 bound specifically,  in vitro  , to a region upstream of the  ava4025  promoter.

AB - A nabaena variabilis  &emsp14;ATCC 29413 fixes nitrogen in specialized cells called heterocysts using either a Mo &hyphen;nitrogenase or a V &hyphen;nitrogenase. V &hyphen;nitrogenase structural genes,  vnfDGK  , as well as  vnfEN  form an operon with  ava4025  , located upstream of  vnfDG  that is repressed by fixed nitrogen and by Mo . The  ava4025  &hyphen; vnfDGKEN  operon is under the control of a Mo &hyphen;repressible promoter located nearly 600 bp upstream of  ava4025  . Levels of  vnfDG  transcript were about 500&hyphen;fold higher than  ava4025  , the first gene of the operon. This may be the result of RNA processing at a site 87 bp upstream of  vnfDG  that was initially identified as the transcription start site. A strain with a deletion in the coding region of  ava4025  grew diazotrophically with Mo or with V . Two similar proteins, VnfR 1 and VnfR 2, whose genes are located some distance from the  ava4025  &hyphen; vnfDGKEN  operon, each repressed transcription from the  ava4025  &hyphen; vnfDGKEN  promoter and a mutant lacking both VnfR 1 and VnfR 2 made the V &hyphen;nitrogenase in the presence of Mo . Overexpression of the V &hyphen;nitrogenase in the double  vnfR1 vnfR2  mutant resulted in decreased activity of the Mo &hyphen;nitrogenase. VnfR 1 bound specifically,  in vitro  , to a region upstream of the  ava4025  promoter.

UR - http://onlinelibrary.wiley.com/doi/10.1111/mmi.12197/abstract

U2 - 10.1111/mmi.12197

DO - 10.1111/mmi.12197

M3 - Article

VL - 88

JO - Molecular Microbiology

JF - Molecular Microbiology

ER -

Regulation of V‐nitrogenase genes in Anabaena variabilis by RNA processing and by dual repressors

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