Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry

Xuemin Wang

doi:10.1038/nprot.2010.37

Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry

Xuemin Wang

University of Missouri-St. Louis

Research output: Contribution to journal › Article › peer-review

Abstract

The ability to measure plant hormones quantitatively is important as plant hormones regulate plant growth, development and response to biotic and abiotic cues. In this protocol, we describe the quantitative analysis of major plant hormones from crude plant extracts. Plant hormones are determined using reverse-phase liquid chromatography–tandem mass spectrometry with multiple reaction monitoring. The method provides quantification of most major plant hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2–3 d.

Original language	American English
Journal	Nature Protocols
Volume	5
DOIs	https://doi.org/10.1038/nprot.2010.37
State	Published - 2010

Disciplines

Botany

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abstract = " The ability to measure plant hormones quantitatively is important as plant hormones regulate plant growth, development and response to biotic and abiotic cues. In this protocol, we describe the quantitative analysis of major plant hormones from crude plant extracts. Plant hormones are determined using reverse-phase liquid chromatography–tandem mass spectrometry with multiple reaction monitoring. The method provides quantification of most major plant hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2–3 d. ",

author = "Xuemin Wang",

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PY - 2010

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AB - The ability to measure plant hormones quantitatively is important as plant hormones regulate plant growth, development and response to biotic and abiotic cues. In this protocol, we describe the quantitative analysis of major plant hormones from crude plant extracts. Plant hormones are determined using reverse-phase liquid chromatography–tandem mass spectrometry with multiple reaction monitoring. The method provides quantification of most major plant hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2–3 d.

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M3 - Article

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JO - Nature Protocols

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Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry

Abstract

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