TY - JOUR
T1 - Proposed Benchmark Methods for Analyzing Acai (Euterpe oleraceae Mart.)
AU - Smith, Robert
AU - Eaker, Janete
AU - Tran, Kevin
AU - Smith, Cynthia
AU - Monroe, Douglas M
AU - da Silva Menezes, Ellen M
AU - Sabaa-Srur, Armando U. O.
AU - Luo, Rensheng
AU - Wycoff, Wei
AU - Fales, William
N1 - Journal Name: The Natural Products Journal Volume 2 , Issue 2 , 2012 Abstract: Methods are proposed for analyzing acai. They start with gravimetric methods for total lipids and insoluble solids, 1H-NMR to determine the relative distribution of the types of lipids, CPMAS 13C-NMR to analyze insoluble solids, and ICP-AES to measure the amounts of total Ca, Mg, K and P.
PY - 2012
Y1 - 2012
N2 - Methods are proposed for analyzing acai. They start with gravimetric methods for total lipids and insoluble solids, 1H-NMR to determine the relative distribution of the types of lipids, CPMAS 13C-NMR to analyze insoluble solids, and ICP-AES to measure the amounts of total Ca, Mg, K and P. The ratio of K to Ca can be used to distinguish between acai products and grape juice. These methods were used to analyze samples. The total lipids were 46.7±2.5%, as triglycerides. The triglycerides contained 24.8±2.8% saturated fatty acyls, 66.1±2.8% mono-, 7.58±0.52% di- and 1.46±0.05% omega-3 unsaturated fatty acyls. A CPMAS 13C-NMR spectrum of the insoluble solids contained peaks due to C=O and/or phenolic carbons, aromatic carbons, glycosidic carbons and aliphatic carbons, with chemical shifts and peak sizes that were different from those of other types of insoluble fibers. HPLC and LC–MS/MS found cyanidin-3-Oglucoside and cyanidin-3-O-rutinoside. Anion chromatography found lactate, acetate, formate, galacturonate, chloride, sulfate, malate, oxalate, phosphate, inositol hexaphosphate (phytic acid), citrate and isocitrate. No Salmonella, coliform bacteria, pesticides, herbicides, fungicides or industrial chemicals were found in genuine Brazilian acai.
AB - Methods are proposed for analyzing acai. They start with gravimetric methods for total lipids and insoluble solids, 1H-NMR to determine the relative distribution of the types of lipids, CPMAS 13C-NMR to analyze insoluble solids, and ICP-AES to measure the amounts of total Ca, Mg, K and P. The ratio of K to Ca can be used to distinguish between acai products and grape juice. These methods were used to analyze samples. The total lipids were 46.7±2.5%, as triglycerides. The triglycerides contained 24.8±2.8% saturated fatty acyls, 66.1±2.8% mono-, 7.58±0.52% di- and 1.46±0.05% omega-3 unsaturated fatty acyls. A CPMAS 13C-NMR spectrum of the insoluble solids contained peaks due to C=O and/or phenolic carbons, aromatic carbons, glycosidic carbons and aliphatic carbons, with chemical shifts and peak sizes that were different from those of other types of insoluble fibers. HPLC and LC–MS/MS found cyanidin-3-Oglucoside and cyanidin-3-O-rutinoside. Anion chromatography found lactate, acetate, formate, galacturonate, chloride, sulfate, malate, oxalate, phosphate, inositol hexaphosphate (phytic acid), citrate and isocitrate. No Salmonella, coliform bacteria, pesticides, herbicides, fungicides or industrial chemicals were found in genuine Brazilian acai.
KW - Acai
KW - NMR
KW - aroma
KW - calcium
KW - ion chromatography
KW - magnsium
KW - phytic acid
KW - potassium
KW - pulp
KW - triglycerides
UR - https://www.eurekaselect.com/99027/article
U2 - 10.2174/2210315511202020076
DO - 10.2174/2210315511202020076
M3 - Article
VL - 2
JO - The Natural Products Journal
JF - The Natural Products Journal
ER -