Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

Xuemin Wang

doi:10.1111/pce.13437

Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

Xuemin Wang

University of Missouri-St. Louis

Research output: Contribution to journal › Article › peer-review

Abstract

<div class="line" id="line-15"> Phosphatidylinositol&hyphen;specific phospholipase C (PI&hyphen;PLC) is involved in stress signalling but its signalling function remains largely unknown in crop plants. Here, we report that the PI&hyphen;PLC4 from rice ( Oryza sativa cv  ), OsPLC4, plays a positive role in osmotic stress response. Two independent knockout mutants,  plc4&hyphen;1  and  plc4&hyphen;2  , exhibited decreased seedling growth and survival rate whereas overexpression of  OsPLC4  improved survival rate under high salinity and water deficiency, compared with wild type (WT). OsPLC4 hydrolyses PI, phosphatidylinositol 4&hyphen;phosphate (PI4P), and phosphatidylinositol&hyphen;4,5&hyphen;bisphosphate (PIP 2 ) to generate diacylglycerol (DAG) in vitro. Knockout of  OsPLC4  attenuated salt&hyphen;induced increase of phosphatidic acid (PA) whereas overexpression of  OsPLC4  decreased the level of PI4P and PIP 2  under salt treatment. Applications of DAG or PA restored the growth defect of  plc4&hyphen;1  to WT but DAG kinase inhibitor 1 blocked the complementary effect of DAG in  plc4&hyphen;1  under salt stress. In addition, the loss of  OsPLC4  compromised the increase of inositol triphosphate and free cytoplasmic Ca 2+  ([Ca 2+ ] cyt ) and inhibited the induction of genes involved in Ca 2+  sensor and osmotic stress response to salt stress. The results indicate that OsPLC4 modulates the activity of two signalling pathways, PA and Ca 2+ , to affect rice seedling response to osmotic stress. </div>

Original language	American English
Journal	Plant, Cell & Environment
DOIs	https://doi.org/10.1111/pce.13437
State	Published - 2018

Keywords

cytoplasmic Ca2+
osmotic stress
phosphatidic acid
phosphatidylinositol‐specific phospholipase C

Disciplines

Botany

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10.1111/pce.13437License: Unspecified

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title = "Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought",

abstract = " Phosphatidylinositol&hyphen;specific phospholipase C (PI&hyphen;PLC) is involved in stress signalling but its signalling function remains largely unknown in crop plants. Here, we report that the PI&hyphen;PLC4 from rice ( Oryza sativa cv  ), OsPLC4, plays a positive role in osmotic stress response. Two independent knockout mutants,  plc4&hyphen;1  and  plc4&hyphen;2  , exhibited decreased seedling growth and survival rate whereas overexpression of  OsPLC4  improved survival rate under high salinity and water deficiency, compared with wild type (WT). OsPLC4 hydrolyses PI, phosphatidylinositol 4&hyphen;phosphate (PI4P), and phosphatidylinositol&hyphen;4,5&hyphen;bisphosphate (PIP 2 ) to generate diacylglycerol (DAG) in vitro. Knockout of  OsPLC4  attenuated salt&hyphen;induced increase of phosphatidic acid (PA) whereas overexpression of  OsPLC4  decreased the level of PI4P and PIP 2  under salt treatment. Applications of DAG or PA restored the growth defect of  plc4&hyphen;1  to WT but DAG kinase inhibitor 1 blocked the complementary effect of DAG in  plc4&hyphen;1  under salt stress. In addition, the loss of  OsPLC4  compromised the increase of inositol triphosphate and free cytoplasmic Ca 2+  ([Ca 2+ ] cyt ) and inhibited the induction of genes involved in Ca 2+  sensor and osmotic stress response to salt stress. The results indicate that OsPLC4 modulates the activity of two signalling pathways, PA and Ca 2+ , to affect rice seedling response to osmotic stress. ",

keywords = "cytoplasmic Ca2+, osmotic stress, phosphatidic acid, phosphatidylinositol‐specific phospholipase C",

author = "Xuemin Wang",

note = "High salinity is a major stress challenging rice production. This study shows that the phosphatidylinositol‐hydrolyzing phospholipase C4 is involved in rice response to NaCl. OsPLC4 modulates the str...",

year = "2018",

doi = "10.1111/pce.13437",

language = "American English",

journal = "Plant, Cell & Environment",

}

TY - JOUR

T1 - Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

AU - Wang, Xuemin

N1 - High salinity is a major stress challenging rice production. This study shows that the phosphatidylinositol‐hydrolyzing phospholipase C4 is involved in rice response to NaCl. OsPLC4 modulates the str...

PY - 2018

Y1 - 2018

N2 - Phosphatidylinositol&hyphen;specific phospholipase C (PI&hyphen;PLC) is involved in stress signalling but its signalling function remains largely unknown in crop plants. Here, we report that the PI&hyphen;PLC4 from rice ( Oryza sativa cv  ), OsPLC4, plays a positive role in osmotic stress response. Two independent knockout mutants,  plc4&hyphen;1  and  plc4&hyphen;2  , exhibited decreased seedling growth and survival rate whereas overexpression of  OsPLC4  improved survival rate under high salinity and water deficiency, compared with wild type (WT). OsPLC4 hydrolyses PI, phosphatidylinositol 4&hyphen;phosphate (PI4P), and phosphatidylinositol&hyphen;4,5&hyphen;bisphosphate (PIP 2 ) to generate diacylglycerol (DAG) in vitro. Knockout of  OsPLC4  attenuated salt&hyphen;induced increase of phosphatidic acid (PA) whereas overexpression of  OsPLC4  decreased the level of PI4P and PIP 2  under salt treatment. Applications of DAG or PA restored the growth defect of  plc4&hyphen;1  to WT but DAG kinase inhibitor 1 blocked the complementary effect of DAG in  plc4&hyphen;1  under salt stress. In addition, the loss of  OsPLC4  compromised the increase of inositol triphosphate and free cytoplasmic Ca 2+  ([Ca 2+ ] cyt ) and inhibited the induction of genes involved in Ca 2+  sensor and osmotic stress response to salt stress. The results indicate that OsPLC4 modulates the activity of two signalling pathways, PA and Ca 2+ , to affect rice seedling response to osmotic stress.

AB - Phosphatidylinositol&hyphen;specific phospholipase C (PI&hyphen;PLC) is involved in stress signalling but its signalling function remains largely unknown in crop plants. Here, we report that the PI&hyphen;PLC4 from rice ( Oryza sativa cv  ), OsPLC4, plays a positive role in osmotic stress response. Two independent knockout mutants,  plc4&hyphen;1  and  plc4&hyphen;2  , exhibited decreased seedling growth and survival rate whereas overexpression of  OsPLC4  improved survival rate under high salinity and water deficiency, compared with wild type (WT). OsPLC4 hydrolyses PI, phosphatidylinositol 4&hyphen;phosphate (PI4P), and phosphatidylinositol&hyphen;4,5&hyphen;bisphosphate (PIP 2 ) to generate diacylglycerol (DAG) in vitro. Knockout of  OsPLC4  attenuated salt&hyphen;induced increase of phosphatidic acid (PA) whereas overexpression of  OsPLC4  decreased the level of PI4P and PIP 2  under salt treatment. Applications of DAG or PA restored the growth defect of  plc4&hyphen;1  to WT but DAG kinase inhibitor 1 blocked the complementary effect of DAG in  plc4&hyphen;1  under salt stress. In addition, the loss of  OsPLC4  compromised the increase of inositol triphosphate and free cytoplasmic Ca 2+  ([Ca 2+ ] cyt ) and inhibited the induction of genes involved in Ca 2+  sensor and osmotic stress response to salt stress. The results indicate that OsPLC4 modulates the activity of two signalling pathways, PA and Ca 2+ , to affect rice seedling response to osmotic stress.

KW - cytoplasmic Ca2+

KW - osmotic stress

KW - phosphatidic acid

KW - phosphatidylinositol‐specific phospholipase C

UR - https://doi.org/10.1111/pce.13437

U2 - 10.1111/pce.13437

DO - 10.1111/pce.13437

M3 - Article

JO - Plant, Cell & Environment

JF - Plant, Cell & Environment

ER -

Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

Abstract

Keywords

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