Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis.

Hieu Sy Vu; Sunitha Shiva; Mary R. Roth; Pamela Tamura; Lianqing Zheng; Maoyin Li; Maoyin Li; Sujon Sarowar; Samuel Honey; Dedan McEllhiney; Paul Hinkes; Lawrence Seib; Todd D. Williams; Gary Gadbury; Xuemin Wang; Xuemin Wang; Jyoti Shah; Ruth Welti

doi:10.1111/TPJ.12659

Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis.

Hieu Sy Vu, Sunitha Shiva, Mary R. Roth, Pamela Tamura, Lianqing Zheng, Maoyin Li, Maoyin Li, Sujon Sarowar, Samuel Honey, Dedan McEllhiney, Paul Hinkes, Lawrence Seib, Todd D. Williams, Gary Gadbury, Xuemin Wang, Xuemin Wang, Jyoti Shah, Ruth Welti

University of Missouri-St. Louis

Research output: Contribution to journal › Article › peer-review

Abstract

<div class="line" id="line-15"> A direct&hyphen;infusion electrospray ionization triple–quadrupole mass spectrometry method with multiple reaction monitoring (MRM ) was employed to measure 264 lipid analytes extracted from leaves of  Arabidopsis thaliana  subjected to mechanical wounding. The method provided precise measurements with an average coefficient of variation of 6.1%. Lipid classes analyzed comprised galactolipids and phospholipids (including monoacyl molecular species, molecular species with oxidized acyl chains, phosphatidic acids (PA s)), tri&hyphen; and tetra&hyphen;galactosyldiacylglycerols (T rGDG s and TeGDG s), head&hyphen;group&hyphen;acylated galactolipids, and head&hyphen;group&hyphen;acylated phosphatidylglycerol (acPG ), sulfoquinovosyldiacylglycerols (SQDG s), sphingolipids, di&hyphen; and tri&hyphen;acylglycerols (DAG s and TAG s), and sterol derivatives. Of the 264 lipid analytes, 254 changed significantly in response to wounding. In general, levels of structural lipids decreased, whereas monoacyl molecular species, galactolipids and phosphatidylglycerols (PG s) with oxidized fatty acyl chains, PA s, TrGDG s, TeGDG s, TAG s, head&hyphen;group&hyphen;acylated galactolipids, acPG , and some sterol derivatives increased, many transiently. The observed changes are consistent with activation of lipid oxidizing, hydrolyzing, glycosylating, and acylating activities in the wounding response. Correlation analysis of the levels of lipid analytes across individual control and treated plants was used to construct a lipid dendrogram and to define clusters and sub&hyphen;clusters of lipid analytes, each composed of a group of lipids which occurred in a coordinated manner. Current knowledge of metabolism supports the notion that observed sub&hyphen;clusters comprise lipids generated by a common enzyme and/or metabolically downstream of a common enzyme. This work demonstrates that co&hyphen;occurrence analysis, based on correlation of lipid levels among plants, is a powerful approach to defining lipids generated  in vivo  by a common enzymatic pathway. </div>

Original language	American English
Journal	Plant Journal
Volume	80
DOIs	https://doi.org/10.1111/TPJ.12659
State	Published - Nov 1 2014

Keywords

Arabidopsis thaliana
Spearman's correlation
dendrogram
direct‐infusion triple–quadrupole mass spectrometry
glycerolipids
leaf wounding
lipid clustering
lipidomics
oxidized lipids
sterol derivatives
technical advance

Disciplines

Biology
Biochemistry

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Vu, H. S., Shiva, S., Roth, M. R., Tamura, P., Zheng, L., Li, M., Li, M., Sarowar, S., Honey, S., McEllhiney, D., Hinkes, P., Seib, L., Williams, T. D., Gadbury, G., Wang, X., Wang, X., Shah, J., & Welti, R. (2014). Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis. Plant Journal, 80. https://doi.org/10.1111/TPJ.12659

Vu, HS, Shiva, S, Roth, MR, Tamura, P, Zheng, L, Li, M, Li, M, Sarowar, S, Honey, S, McEllhiney, D, Hinkes, P, Seib, L, Williams, TD, Gadbury, G, Wang, X , Wang, X, Shah, J & Welti, R 2014, 'Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis.', Plant Journal, vol. 80. https://doi.org/10.1111/TPJ.12659

@article{9f826cfdb9884f1e9c7b3edb427a1153,

title = "Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis.",

abstract = " A direct&hyphen;infusion electrospray ionization triple–quadrupole mass spectrometry method with multiple reaction monitoring (MRM ) was employed to measure 264 lipid analytes extracted from leaves of  Arabidopsis thaliana  subjected to mechanical wounding. The method provided precise measurements with an average coefficient of variation of 6.1%. Lipid classes analyzed comprised galactolipids and phospholipids (including monoacyl molecular species, molecular species with oxidized acyl chains, phosphatidic acids (PA s)), tri&hyphen; and tetra&hyphen;galactosyldiacylglycerols (T rGDG s and TeGDG s), head&hyphen;group&hyphen;acylated galactolipids, and head&hyphen;group&hyphen;acylated phosphatidylglycerol (acPG ), sulfoquinovosyldiacylglycerols (SQDG s), sphingolipids, di&hyphen; and tri&hyphen;acylglycerols (DAG s and TAG s), and sterol derivatives. Of the 264 lipid analytes, 254 changed significantly in response to wounding. In general, levels of structural lipids decreased, whereas monoacyl molecular species, galactolipids and phosphatidylglycerols (PG s) with oxidized fatty acyl chains, PA s, TrGDG s, TeGDG s, TAG s, head&hyphen;group&hyphen;acylated galactolipids, acPG , and some sterol derivatives increased, many transiently. The observed changes are consistent with activation of lipid oxidizing, hydrolyzing, glycosylating, and acylating activities in the wounding response. Correlation analysis of the levels of lipid analytes across individual control and treated plants was used to construct a lipid dendrogram and to define clusters and sub&hyphen;clusters of lipid analytes, each composed of a group of lipids which occurred in a coordinated manner. Current knowledge of metabolism supports the notion that observed sub&hyphen;clusters comprise lipids generated by a common enzyme and/or metabolically downstream of a common enzyme. This work demonstrates that co&hyphen;occurrence analysis, based on correlation of lipid levels among plants, is a powerful approach to defining lipids generated  in vivo  by a common enzymatic pathway. ",

keywords = "Arabidopsis thaliana, Spearman's correlation, dendrogram, direct‐infusion triple–quadrupole mass spectrometry, glycerolipids, leaf wounding, lipid clustering, lipidomics, oxidized lipids, sterol derivatives, technical advance",

author = "Vu, {Hieu Sy} and Sunitha Shiva and Roth, {Mary R.} and Pamela Tamura and Lianqing Zheng and Maoyin Li and Maoyin Li and Sujon Sarowar and Samuel Honey and Dedan McEllhiney and Paul Hinkes and Lawrence Seib and Williams, {Todd D.} and Gary Gadbury and Xuemin Wang and Xuemin Wang and Jyoti Shah and Ruth Welti",

note = "A direct‐infusion electrospray ionization triple-quadrupole mass spectrometry method with multiple reaction monitoring (MRM ) was employed to measure 264 lipid analytes extracted from leaves of Arabidopsis thaliana subjected to mechanical wounding. The method provided precise measurements with an average coefficient of variation of 6.1%.",

year = "2014",

month = nov,

day = "1",

doi = "10.1111/TPJ.12659",

language = "American English",

volume = "80",

journal = "Plant Journal",

}

TY - JOUR

T1 - Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis.

AU - Vu, Hieu Sy

AU - Shiva, Sunitha

AU - Roth, Mary R.

AU - Tamura, Pamela

AU - Zheng, Lianqing

AU - Li, Maoyin

AU - Sarowar, Sujon

AU - Honey, Samuel

AU - McEllhiney, Dedan

AU - Hinkes, Paul

AU - Seib, Lawrence

AU - Williams, Todd D.

AU - Gadbury, Gary

AU - Wang, Xuemin

AU - Shah, Jyoti

AU - Welti, Ruth

N1 - A direct‐infusion electrospray ionization triple-quadrupole mass spectrometry method with multiple reaction monitoring (MRM ) was employed to measure 264 lipid analytes extracted from leaves of Arabidopsis thaliana subjected to mechanical wounding. The method provided precise measurements with an average coefficient of variation of 6.1%.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - A direct&hyphen;infusion electrospray ionization triple–quadrupole mass spectrometry method with multiple reaction monitoring (MRM ) was employed to measure 264 lipid analytes extracted from leaves of  Arabidopsis thaliana  subjected to mechanical wounding. The method provided precise measurements with an average coefficient of variation of 6.1%. Lipid classes analyzed comprised galactolipids and phospholipids (including monoacyl molecular species, molecular species with oxidized acyl chains, phosphatidic acids (PA s)), tri&hyphen; and tetra&hyphen;galactosyldiacylglycerols (T rGDG s and TeGDG s), head&hyphen;group&hyphen;acylated galactolipids, and head&hyphen;group&hyphen;acylated phosphatidylglycerol (acPG ), sulfoquinovosyldiacylglycerols (SQDG s), sphingolipids, di&hyphen; and tri&hyphen;acylglycerols (DAG s and TAG s), and sterol derivatives. Of the 264 lipid analytes, 254 changed significantly in response to wounding. In general, levels of structural lipids decreased, whereas monoacyl molecular species, galactolipids and phosphatidylglycerols (PG s) with oxidized fatty acyl chains, PA s, TrGDG s, TeGDG s, TAG s, head&hyphen;group&hyphen;acylated galactolipids, acPG , and some sterol derivatives increased, many transiently. The observed changes are consistent with activation of lipid oxidizing, hydrolyzing, glycosylating, and acylating activities in the wounding response. Correlation analysis of the levels of lipid analytes across individual control and treated plants was used to construct a lipid dendrogram and to define clusters and sub&hyphen;clusters of lipid analytes, each composed of a group of lipids which occurred in a coordinated manner. Current knowledge of metabolism supports the notion that observed sub&hyphen;clusters comprise lipids generated by a common enzyme and/or metabolically downstream of a common enzyme. This work demonstrates that co&hyphen;occurrence analysis, based on correlation of lipid levels among plants, is a powerful approach to defining lipids generated  in vivo  by a common enzymatic pathway.

AB - A direct&hyphen;infusion electrospray ionization triple–quadrupole mass spectrometry method with multiple reaction monitoring (MRM ) was employed to measure 264 lipid analytes extracted from leaves of  Arabidopsis thaliana  subjected to mechanical wounding. The method provided precise measurements with an average coefficient of variation of 6.1%. Lipid classes analyzed comprised galactolipids and phospholipids (including monoacyl molecular species, molecular species with oxidized acyl chains, phosphatidic acids (PA s)), tri&hyphen; and tetra&hyphen;galactosyldiacylglycerols (T rGDG s and TeGDG s), head&hyphen;group&hyphen;acylated galactolipids, and head&hyphen;group&hyphen;acylated phosphatidylglycerol (acPG ), sulfoquinovosyldiacylglycerols (SQDG s), sphingolipids, di&hyphen; and tri&hyphen;acylglycerols (DAG s and TAG s), and sterol derivatives. Of the 264 lipid analytes, 254 changed significantly in response to wounding. In general, levels of structural lipids decreased, whereas monoacyl molecular species, galactolipids and phosphatidylglycerols (PG s) with oxidized fatty acyl chains, PA s, TrGDG s, TeGDG s, TAG s, head&hyphen;group&hyphen;acylated galactolipids, acPG , and some sterol derivatives increased, many transiently. The observed changes are consistent with activation of lipid oxidizing, hydrolyzing, glycosylating, and acylating activities in the wounding response. Correlation analysis of the levels of lipid analytes across individual control and treated plants was used to construct a lipid dendrogram and to define clusters and sub&hyphen;clusters of lipid analytes, each composed of a group of lipids which occurred in a coordinated manner. Current knowledge of metabolism supports the notion that observed sub&hyphen;clusters comprise lipids generated by a common enzyme and/or metabolically downstream of a common enzyme. This work demonstrates that co&hyphen;occurrence analysis, based on correlation of lipid levels among plants, is a powerful approach to defining lipids generated  in vivo  by a common enzymatic pathway.

KW - Arabidopsis thaliana

KW - Spearman's correlation

KW - dendrogram

KW - direct‐infusion triple–quadrupole mass spectrometry

KW - glycerolipids

KW - leaf wounding

KW - lipid clustering

KW - lipidomics

KW - oxidized lipids

KW - sterol derivatives

KW - technical advance

UR - https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12659

U2 - 10.1111/TPJ.12659

DO - 10.1111/TPJ.12659

M3 - Article

VL - 80

JO - Plant Journal

JF - Plant Journal

ER -

Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis.

Abstract

Keywords

Disciplines

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