Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina

Guangqin Cai; Geliang Wang; Sang-Chul Kim; Jianwu Li; Yongming Zhou; Xuemin Wang

doi:10.1186/S13068-021-01899-W

Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina

Guangqin Cai, Geliang Wang, Sang-Chul Kim, Jianwu Li, Yongming Zhou, Xuemin Wang

University of Missouri-St. Louis

Research output: Contribution to journal › Article › peer-review

Abstract

 Background
 Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied.
 Results
 Here, we examined the effect of two lipid transporters,    FAX1   (   fatty acid export1  ) and    ABCA9   (   ATP-binding cassette transporter subfamily A9  ) on oil production and lipid metabolism in the oilseed plant    Camelina sativa  . Overexpression (OE) of    FAX1   and    ABCA9   increased seed weight and size, with    FAX1  -OEs and    ABCA9  -OEs increasing seed length and width, respectively, whereas    FAX1  /   ABCA9  -OEs increasing both.    FAX1-  OE and    ABCA9-  OE displayed additive effects on seed oil content and seed yield. Also, OE of    FAX1   and    ABCA9   affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as    DGAT2  ,    PDAT1  , and    LEC1  , was increased in developing seeds of    FAX1  - and/or    ABCA9  -OEs.
 Conclusion
 These results indicate that increased expression of    FAX1   and    ABCA9   can potentially be applied to improving camelina oil production.

Original language	American English
Journal	Biotechnology for Biofuels
Volume	14
DOIs	https://doi.org/10.1186/S13068-021-01899-W
State	Published - Feb 2021

Disciplines

Biology

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Cite this

@article{2271dce3c15945cda800f338c283fc89,

title = "Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina",

abstract = " Background Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. Results Here, we examined the effect of two lipid transporters, FAX1 ( fatty acid export1 ) and ABCA9 ( ATP-binding cassette transporter subfamily A9 ) on oil production and lipid metabolism in the oilseed plant Camelina sativa . Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1 -OEs and ABCA9 -OEs increasing seed length and width, respectively, whereas FAX1 / ABCA9 -OEs increasing both. FAX1- OE and ABCA9- OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2 , PDAT1 , and LEC1 , was increased in developing seeds of FAX1 - and/or ABCA9 -OEs. Conclusion These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.",

author = "Guangqin Cai and Geliang Wang and Sang-Chul Kim and Jianwu Li and Yongming Zhou and Xuemin Wang",

year = "2021",

month = feb,

doi = "10.1186/S13068-021-01899-W",

language = "American English",

volume = "14",

journal = "Biotechnology for Biofuels",

}

TY - JOUR

T1 - Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina

AU - Cai, Guangqin

AU - Wang, Geliang

AU - Kim, Sang-Chul

AU - Li, Jianwu

AU - Zhou, Yongming

AU - Wang, Xuemin

PY - 2021/2

Y1 - 2021/2

N2 - Background Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. Results Here, we examined the effect of two lipid transporters, FAX1 ( fatty acid export1 ) and ABCA9 ( ATP-binding cassette transporter subfamily A9 ) on oil production and lipid metabolism in the oilseed plant Camelina sativa . Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1 -OEs and ABCA9 -OEs increasing seed length and width, respectively, whereas FAX1 / ABCA9 -OEs increasing both. FAX1- OE and ABCA9- OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2 , PDAT1 , and LEC1 , was increased in developing seeds of FAX1 - and/or ABCA9 -OEs. Conclusion These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.

AB - Background Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. Results Here, we examined the effect of two lipid transporters, FAX1 ( fatty acid export1 ) and ABCA9 ( ATP-binding cassette transporter subfamily A9 ) on oil production and lipid metabolism in the oilseed plant Camelina sativa . Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1 -OEs and ABCA9 -OEs increasing seed length and width, respectively, whereas FAX1 / ABCA9 -OEs increasing both. FAX1- OE and ABCA9- OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2 , PDAT1 , and LEC1 , was increased in developing seeds of FAX1 - and/or ABCA9 -OEs. Conclusion These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.

U2 - 10.1186/S13068-021-01899-W

DO - 10.1186/S13068-021-01899-W

M3 - Article

VL - 14

JO - Biotechnology for Biofuels

JF - Biotechnology for Biofuels

ER -