TY - JOUR
T1 - Identification of Changes in Gene Expression by Quantitation of mRNA Levels
AU - Olivas, Wendy M.
N1 - Part of the Methods In Molecular Biology™ book series (MIMB, volume 419) Sequence elements within mRNA-untranslated regions and their binding partners are key controllers of mRNA stability. Changes in mRNA stability can often be detected by changes in steady-state mRNA abundance, or a more careful analysis of mRNA half-lives can be performed following transcriptional repression.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Sequence elements within mRNA-untranslated regions and their binding partners are key controllers of mRNA stability. Changes in mRNA stability can often be detected by changes in steady-state mRNA abundance, or a more careful analysis of mRNA half-lives can be performed following transcriptional repression. This chapter presents methods to isolate RNA from both yeast and mammalian cells for either steady-state or half-life analyses. In addition, two reliable methods to quantitate mRNA levels, northern blot analysis and real-time PCR, are outlined and compared.
AB - Sequence elements within mRNA-untranslated regions and their binding partners are key controllers of mRNA stability. Changes in mRNA stability can often be detected by changes in steady-state mRNA abundance, or a more careful analysis of mRNA half-lives can be performed following transcriptional repression. This chapter presents methods to isolate RNA from both yeast and mammalian cells for either steady-state or half-life analyses. In addition, two reliable methods to quantitate mRNA levels, northern blot analysis and real-time PCR, are outlined and compared.
UR - https://rd.springer.com/content/pdf/10.1007%2F978-1-59745-033-1_17.pdf
U2 - 10.1007/978-1-59745-033-1_17
DO - 10.1007/978-1-59745-033-1_17
M3 - Article
VL - 419
JO - Methods of Molecular Biology
JF - Methods of Molecular Biology
ER -