High-Resolution, Long-Term Characterization of Bacterial Motility Using Optical Tweezers

Lon Chubiz; Taejin L. Min; Patrick J. Mears; Christopher V. Rao; Ido Golding; Yann R. Chemla

doi:10.1038/nmeth.1380

High-Resolution, Long-Term Characterization of Bacterial Motility Using Optical Tweezers

Lon Chubiz, Taejin L. Min, Patrick J. Mears, Christopher V. Rao, Ido Golding, Yann R. Chemla

University of Missouri-St. Louis

Research output: Contribution to journal › Article › peer-review

Abstract

We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution greater than the flagellar rotation rates of ∼100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction.

Original language	American English
Journal	Nature Methods
Volume	6
DOIs	https://doi.org/10.1038/nmeth.1380
State	Published - 2009

Disciplines

Biology

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Cite this

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title = "High-Resolution, Long-Term Characterization of Bacterial Motility Using Optical Tweezers",

abstract = " We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution greater than the flagellar rotation rates of ∼100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction.",

author = "Lon Chubiz and Min, {Taejin L.} and Mears, {Patrick J.} and Rao, {Christopher V.} and Ido Golding and Chemla, {Yann R.}",

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language = "American English",

volume = "6",

journal = "Nature Methods",

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AU - Chubiz, Lon

AU - Min, Taejin L.

AU - Mears, Patrick J.

AU - Rao, Christopher V.

AU - Golding, Ido

AU - Chemla, Yann R.

PY - 2009

Y1 - 2009

N2 - We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution greater than the flagellar rotation rates of ∼100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction.

AB - We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution greater than the flagellar rotation rates of ∼100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction.

U2 - 10.1038/nmeth.1380

DO - 10.1038/nmeth.1380

M3 - Article

VL - 6

JO - Nature Methods

JF - Nature Methods

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