Comparative Study of the Binding of Concanavalin A to Self-Assembled Monolayers Containing a Thiolated α-Mannoside on Flat Gold and on Nanoporous Gold

Binod Pandey; Yih Horng Tan; Kohki Fujikawa; Alexei V. Demchenko; Keith J. Stine

doi:10.1080/07328303.2012.683909

Comparative Study of the Binding of Concanavalin A to Self-Assembled Monolayers Containing a Thiolated α-Mannoside on Flat Gold and on Nanoporous Gold

Binod Pandey, Yih Horng Tan, Kohki Fujikawa, Alexei V. Demchenko, Keith J. Stine

University of Missouri-St. Louis

Research output: Contribution to journal › Article › peer-review

Abstract

We have prepared SAMs containing 8-mercaptooctyl α-D-mannopyranoside, either as a single component or in mixed SAMs with n-octanethiol on flat gold surfaces and on nanoporous gold. Electrochemical impedance spectroscopy showed that the mixed SAMs on flat gold surfaces showed the highest Con A binding near 1:9 solution molar ratio of thiolatedα-mannoside to n-octanethiol whereas those on NPG showed the highest response at 1:19 solution molar ratio of thiolated α-mannoside to n-octanethiol. Atomic force microscopy was employed to image the monolayers, and also to image the bound Con A protein.

Original language	American English
Journal	Journal of Carbohydrate Chemistry
Volume	31
DOIs	https://doi.org/10.1080/07328303.2012.683909
State	Published - May 1 2012

Disciplines

Organic Chemistry
Biochemistry

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title = "Comparative Study of the Binding of Concanavalin A to Self-Assembled Monolayers Containing a Thiolated α-Mannoside on Flat Gold and on Nanoporous Gold",

abstract = " We have prepared SAMs containing 8-mercaptooctyl α-D-mannopyranoside, either as a single component or in mixed SAMs with n-octanethiol on flat gold surfaces and on nanoporous gold. Electrochemical impedance spectroscopy showed that the mixed SAMs on flat gold surfaces showed the highest Con A binding near 1:9 solution molar ratio of thiolatedα-mannoside to n-octanethiol whereas those on NPG showed the highest response at 1:19 solution molar ratio of thiolated α-mannoside to n-octanethiol. Atomic force microscopy was employed to image the monolayers, and also to image the bound Con A protein.",

author = "Binod Pandey and Tan, {Yih Horng} and Kohki Fujikawa and Demchenko, {Alexei V.} and Stine, {Keith J.}",

note = "Carbohydrates are among the most complex and prevalent biomolecules in living organisms. Cell surfaces are highly decorated with carbohydrates presented in the form of different glycoconjugates including glycolipids, glycoproteins, glycosaminoglycans and proteoglycans. Glycosylation patterns differ according to cell type, stage of cell development, growth, and differentiation.",

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T1 - Comparative Study of the Binding of Concanavalin A to Self-Assembled Monolayers Containing a Thiolated α-Mannoside on Flat Gold and on Nanoporous Gold

AU - Pandey, Binod

AU - Tan, Yih Horng

AU - Fujikawa, Kohki

AU - Demchenko, Alexei V.

AU - Stine, Keith J.

N1 - Carbohydrates are among the most complex and prevalent biomolecules in living organisms. Cell surfaces are highly decorated with carbohydrates presented in the form of different glycoconjugates including glycolipids, glycoproteins, glycosaminoglycans and proteoglycans. Glycosylation patterns differ according to cell type, stage of cell development, growth, and differentiation.

PY - 2012/5/1

Y1 - 2012/5/1

N2 - We have prepared SAMs containing 8-mercaptooctyl α-D-mannopyranoside, either as a single component or in mixed SAMs with n-octanethiol on flat gold surfaces and on nanoporous gold. Electrochemical impedance spectroscopy showed that the mixed SAMs on flat gold surfaces showed the highest Con A binding near 1:9 solution molar ratio of thiolatedα-mannoside to n-octanethiol whereas those on NPG showed the highest response at 1:19 solution molar ratio of thiolated α-mannoside to n-octanethiol. Atomic force microscopy was employed to image the monolayers, and also to image the bound Con A protein.

AB - We have prepared SAMs containing 8-mercaptooctyl α-D-mannopyranoside, either as a single component or in mixed SAMs with n-octanethiol on flat gold surfaces and on nanoporous gold. Electrochemical impedance spectroscopy showed that the mixed SAMs on flat gold surfaces showed the highest Con A binding near 1:9 solution molar ratio of thiolatedα-mannoside to n-octanethiol whereas those on NPG showed the highest response at 1:19 solution molar ratio of thiolated α-mannoside to n-octanethiol. Atomic force microscopy was employed to image the monolayers, and also to image the bound Con A protein.

UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601678/

U2 - 10.1080/07328303.2012.683909

DO - 10.1080/07328303.2012.683909

M3 - Article

VL - 31

JO - Journal of Carbohydrate Chemistry

JF - Journal of Carbohydrate Chemistry

ER -

Comparative Study of the Binding of Concanavalin A to Self-Assembled Monolayers Containing a Thiolated α-Mannoside on Flat Gold and on Nanoporous Gold

Abstract

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